Kyrgios was facing Casper Ruud in the event in Rome and had fought back to level the match at one set all.
He was a break up in the third though hit a forehand wide to hand the break back to his opponent.
And the Australian clearly wasn’t happy with someone in the crowd as he shouted towards the spectators.
Kyrgios began his rant by shouting: “F***ing r****d bro.”
The umpire had already handed Kyrgios multiple code violations earlier in the match and the 24-year-old was clearly agitated.
He continued to the crowd saying: “You’re not supposed to walk across the court as I’m serving, do you understand that?
“It’s b********, I don’t want to play when this s**t is going on.”
That’s when Kyrgios smashes his racket before kicking a bottle and then launching a chair onto the court.
And the Australian’s rant continues as he packed up his bag and walked off the court, leading to his default.
He ranted: “I’m giving 110 [per cent] and having to deal with fucking idiots like him.
“I’m done, I’m f***ing done. F***ing done with it.”
It’s not the first time Kyrgios has shown his anger on the court.
During the Miami Open in March Kyrigos also got involved in a heated altercation with a fan.
Kyrgios’s Italian Open antics came just 24 hours after he took aim at world No 1 Novak Djokovic.
Kyrgios slammed the Serbian saying: “I just feel like he’s got a sick obsession with wanting to be liked.
“He just wants to be Federer. I feel he wants to be liked so much that I just can’t stand him. This celebration thing is so cringeworthy.
“He’s unbelievable, a champion of the sport, one of the greatest we will ever see. I think he will get the Grand Slam count and overtake Federer.
“[But] we’re talking about a guy who pulled out of the Australian Open one year because it was too hot.
“No matter how many Grand Slams he wins, he will never be the greatest to me.”